Journal: BMC Infectious Diseases

Article Title: Development and evaluation of novel Brucella diagnostic antigen protein ST

doi: 10.1186/s12879-025-12393-1

Figure Lengend Snippet: Validation of ST protein expression and purification. A . SDS-PAGE electrophoresis results. M: Marker; Lane 1: Before IPTG induction; Lane 2: Supernatant of 0.5 M IPTG-induced at 37 °C; Lane 3: Supernatant of 1 M IPTG-induced at 37 °C; Lane 4: Supernatant of 0.5 M IPTG-induced at 25 °C; Lane 5: Supernatant of 1 M IPTG-induced at 25 °C; Lane 6: Supernatant of 0.5 M IPTG-induced at 16 °C; Lane 7: Supernatant of 1 M IPTG-induced at 16 °C; Lane 8: Deposition of 0.5 M IPTG-induced at 37 °C; Lane 9: Deposition of 1 M IPTG-induced at 37 °C; Lane 10: Deposition of 0.5 M IPTG-induced at 25 °C; Lane 11: Deposition of 1 M IPTG-induced at 25 °C; Lane 12: Deposition of 0.5 M IPTG-induced at 16 °C; Lane 13: Deposition of 1 M IPTG-induced at 16 °C. B . Western blot (WB) results of purified HIS tag protein. M: Marker; Lane 1: Purified recombinant protein ST protein

Article Snippet: Finally, the purified ST protein was electrophoresed on a 10% SDS-PAGE gel (Bio-Rad, Hercules, USA; Cat. 161–0183), transferred to a PVDF membrane (Millipore, Burlington, USA; Cat. IPVH00010), washed three times with TBST buffer containing 0.05% Tween 20 (Amersham Biosciences, Little Chalfont, UK; Cat. GE2000) for 10 min each time, and subjected to Western blotting with rabbit anti-His tag polyclonal antibody (Proteintech, Rosemont, USA; Cat. 13844-1-AP) and HRP-labeled goat anti-rabbit IgG antibody (Santa Cruz Biotechnology, Dallas, USA; Cat. sc-2004).

Techniques: Biomarker Discovery, Expressing, Purification, SDS Page, Electrophoresis, Marker, Western Blot, Recombinant